Microorganism Biotechnology
Publisher:喻庆勇  Time2016-12-23 View:407

Biotechnological research direction of microbiology is a group specializing in the R&D of microbiology under the leading of Prof. Yang Qian. During the 8th, 9th and 10th Five Years Key Programs for Science and Technology Development of China, this group has undertaken a lot of programs supported by the national and local government, which include 2 projects supported by National Natural Science Foundation of China, 2 projects supported by Ministry of Education of China, 1 project supported by Chinese National Programs for High Technology Research and Development (863 program), 1 Key Program for Science and Technology Development of Heilongjiang province, 3 scientific and technological projects of Heilongjiang province, 1 Science and Technology Cooperation Project of China and Thailand and two local key task projects. Probe into environmental protection, biological control, bioengineering has acquired much highly effective production. In the aspect of biological control of plant disease, the group has cooperated with Dr. Dusanee in King Mongkut’s Institute of Technology Ladkrabang of Thailand since 1994, to explore the mechanisms of biological control of many plant soil-spread diseases by some important fungi such as Chaetomiumglobosum and Trichodermaharzianum,and also to observe the effect of controlling fungi diseases on fruit trees and vegetables. While in gene engineering, the group first successfully transformed the resistant gene ofantiseptic benzimidazole intoChaetomiumglobosum and Trichodermaharzianum. This accomplishment has resolved the problem which explored more than ten years in the world, coming up to international advanced level. In genome research of biological control fungi, high quality cDNA libraries of Chaetomiumglobosum and Trichodermaharzianum during the period of mycelium have already been constructed, and 6505 random-selected clones have been sequenced and high quality expressed sequence tags (EST), logged in Genbank, have been analyzed by means of bioinformatics. Therefore, the accomplishment may offer train of thoughts and tech support for further research of functional genome of other biological control fungi.

The research group have published more than 130 papers and 8 monographs, applied for 5 patents, identified 5 items of research accomplishment, and hosted international conferences for 4 times.


1.Microorganism Fermentation Engineering

  1. Microorganism Genetic Engineering

  2. Microorganism Genomics


  1. Chinese National Natural Science Foundation

Gene Transformation of Microorganisms and Treatment of Solid Castoff (50178021,70,000 yuan,Jan. to Dec., 2002)

  1. Chinese National Programs for High Technology Research and Development

Creation of New Types of Microbial Pesticide, Research of Fermentation Engineering Techniques and Explore of its Production (2003AA241140,1,500,000 yuan,2003-2005)

  1. Key Project of Heilong jiangProvincial Natural Scientific Foundation

Sequences of ESTs in Biological Control Fungi and Study on Expression of Biological Control Genes (ZJN03-04,180,000 yuan,2004-2006)


  1. “Second-class” Advance of Science and Technology in Heilongjiang Province (two items)

  2. “First-class” Advance of Science and Technology of Heilongjiang Education Committee (one item)

  3. “Second-class” Advance of Science and Technology of Heilongjiang Education Committee (one item)

A Brief Introduction of Prof. Yang Qian

Dr. Yang Qian, the committee member of supervisory committee of professional teaching in biotechnology and bioengineering, the director and professional judge of Journal of China Biotechnology, the committee member of Young committee of Chinese Society for Plant Pathology, the ViceChairman of Heilongjiang Society for Plant Pathology, and the initiator of bioengineering specialty of Harbin Institute of Technology, is the professor and doctoral supervisor of Harbin Institute of Technology. Prof. Yang studied in Reading University in British since 1986, where he received the master and the doctorial degree. He has been engaged in the study of plant pathology, biological control of plant diseases, environmental microorganisms, bioengineering and genomes on microorganisms of biological control since his returning in 1991 with doctorial degree. He has directed 2 projects supported by National Natural Science Foundation of China, 2 projects supported by Ministry of Education of the People’s Republic of China, 1 project supported by Chinese National Programs for High Technology Research and Development (863 program), 1 key project supported by natural science foundation of Heilongjiang province, 3 scientific and technological projects of Heilongjiang province. To date, he has published more than 130 papers, which include more than 40 in Science Citation Index and Engineering Index, 1 embodied by Review of Plant Pathology, 32 in international meeting, and 8 scholarly treatises. He was invited to attend international meetings for 7 times, and chaired 4 of them. He is the Visiting Professor of King Mongkut’s Institute of Technology Ladkrabangin Thailand. The international co-operations with them on biological control of plant diseases and the disposal and utilization of solid wastes are now taking place.


  1. Fan J-x, Yang Q, Yao L, et al. Identification and characterization of filamentous fungus during xylose fermentation for ethanol production. Journal of the Harbin Institute of Technology,2011,43(6):65-69.

  2. Fan J-X, Yang X-X, Song J-Z, et al. Heterologous Expression of Transaldolase Gene Tal from Saccharomyces cerevisiae in Fusarium oxysporum for Enhanced Bioethanol Production. Applied Biochemistry And Biotechnology,2011,164(7):1023-1036.

  3. Guang-Li C, Xun-Feng X, Lei Z, et al. Development of AFEX-based consolidated bioprocessing on wheat straw for biohydrogen production using anaerobic microflora. International Journal of Hydrogen Energy,2013,38(35):15653-15659.

  4. Guo Q, Hao J, Yang Q, et al. A comparison of the effectiveness between amniotic membrane homogenate and transplanted amniotic membrane in healing corneal damage in a rabbit model. Acta Ophthalmologica,2011,89(4):E315-E319.

  5. Huang X-M, Yang Q, Liu Z-H, et al. Cloning and Heterologous Expression of a Novel Endoglucanase Gene egVIII from Trichoderma viride in Saccharomyces cerevisiae. Applied Biochemistry And Biotechnology,2010,162(1):103-115.

  6. Liu B, Li Y, Song J, et al. Production of single-cell protein with two-step fermentation for treatment of potato starch processing waste. Cellulose,2014,21(5):3637-3645.

  7. Liu B, Song J, Li Y, et al. Towards Industrially Feasible Treatment of Potato Starch Processing Waste by Mixed Cultures. Applied Biochemistry And Biotechnology,2013,171(4):1001-1010.

  8. Liu Z, Yang X, Sun D, et al. Expressed sequence tags-based identification of genes in a biocontrol strain Trichoderma asperellum. Molecular Biology Reports,2010,37(8):3673-3681.

  9. Odeph M, Qian Y, Phiri J. Molecular cloning of ERG 2 gene to effect knockdown in chaetomium cupreum. 2012 IEEE International Conference on Information Science and Technology,2012,:25-28.

  10. Song J, Liu B, Liu Z, et al. Cloning of two cellobiohydrolase genes from Trichoderma viride and heterogenous expression in yeast Saccharomyces cerevisiae. Molecular Biology Reports,2010,37(4):2135-2140.

  11. Song J, Yang Q, Yang J, et al. Essential Genetic Interactors of SIR2 Required for Spatial Sequestration and Asymmetrical Inheritance of Protein Aggregates. Plos Genetics,2014,10(7).

  12. Wang S, Yang Q, Liu Z-H, et al. Haloterrigena daqingensis sp. nov., an extremely haloalkaliphilic archaeon isolated from a saline-alkaline soil. International Journal Of Systematic and Evolutionary Microbiology,2010,60:2267-2271.

  13. Wang Y, Song J, Wu Y, et al. Eplt4 Proteinaceous Elicitor Produced in Pichia pastoris Has a Protective Effect Against Cercosporidium sofinum Infections of Soybean Leaves. Applied Biochemistry and Biotechnology,2013,169(3):722-737.

  14. Wang Y, Song J-Z, Yang Q, et al. Cloning of a Heat-Stable Chitin Deacetylase Gene from Aspergillus nidulans and its Functional Expression in Escherichia coli. Applied Biochemistry And Biotechnology,2010,162(3):843-854.

  15. Wang Y, Zhao L, Cong H, et al. Optimization of Fermentation Conditions for EplT4 Production by Pichia pastoris Expression System Using Response Surface Methodology. International Journal Of Agriculture And Biology,2013,15(5):850-856.

  16. Wang Z, Cao G, Jiang C, et al. Butanol Production from Wheat Straw by Combining Crude Enzymatic Hydrolysis and Anaerobic Fermentation Using Clostridium acetobutylicum ATCC824. Energy & Fuels,2013,27(10):5900-5906.

  17. Xu J, Yang Q. Isolation and characterization of rice straw degrading Streptomyces griseorubens C-5. Biodegradation,2010,21(1):107-116.

  18. Yao L, Yang Q, Song J, et al. Cloning, Annotation and Expression Analysis of Mycoparasitism-Related Genes in Trichoderma harzianum 88. Journal Of Microbiology,2013,51(2):174-182.

  19. Zhang L-j, Yang Q, Chen Z-x, et al. Degrading Characteristics of Two Petrolecm-degrading Strains. Journal of Nanjing University of Science and Technology,2010,34(6):849-854.