Cancer is a  serious disease threatening to human health; its incidence is on the increase  every year. The research group of the molecular cell biology of cancer focuses  mainly on the expression and epigenetic regulatory mechanism of important genes  which are related with the process of tumor genesis, development and metastasis.  In addition, the group also pays attention to the alteration i n signal  transduction pathway of cancer cells, the structure characteristics and its  mechanism of cancer cells, the glycan structure of cancer cell surface and  cancer metabolomics. The goal of the group is to elucidate the molecular  mechanism of cancer genesis, development and metastasis, and explore new ways  for early diagnosis and treatment of cancer by the development of  transformational medicine technology.

The staff  consists of 1 professor, 4 associated professors and 3 lecturers i n the group.  In addition, 2 postdoc, 8 PhD students and 10 master students are engaged in.  Most of staff member have overseas study or research experience, and have  publishing records with academic value i n key domestic and international  professional journals. The main group members include Li Yu, Shi Shuliang, He  Jie, Tian Weiming, Nie Huan, Han fang, Han Fengtong and Zhang  Hao.
Recent years,  the group has been granted more than 4.9 million and 20 projects including 7  National Natural Science Funds Fund(NSFC), 3 National High-tech Program of China  (863 Program), 2 Major national scientific research plan (973 Program), 3  National Lab projects and 7 other provincial and ministerial level projects.  Over 30 SCI indexed research papers have been published totally.

Teaching  courses:
The teachers of  the group undertake the teaching task on the PhD, master and undergraduate  students including biochemistry, cell biology, molecular biology, immunology,  molecular genetics, molecular cell biology, technique of immunology, molecular  cell biology of cancer, cell engineering, gene engineering, and structure and  function of biomacromolecules. Furthermore the lab is open for all students and  provides the technical support to innovation research.

Student  recruitment:
The group could  enroll Master of Science on the direction of cell biology, biochemistry and  molecular biology i n biology discipline, and Doctor of Science on the direction  of the molecular cell biology of cancer i n biomedical engineering discipline.  Also, could enroll post-doctor.

International  communications
We have  collaborated closely with the Harvard University, School of Medicine, Texas A  and M University i n the area of cancer biology, and Cleveland State University  i n the area of Glycomics. The collaboration includes cooperating training of  graduate students, junior scientist training and collaborative research, and  contract professor engagement.

The  main research fields:
1) The  study on the function and regulation of cancer related gene and  protein
The main  research fields are functional identification and expression regulation of new  genes that are closely related with carcinogenesis. In the aspect of expression  regulation, we focus on the epigenetic regulation mechanism including histone  methylation, histone acetylation, DNA methylation and related regulation.  Meanwhile, the group also conducts researches on the regulation mechanism and  the downstream target of cancer related miRNA. On the other hand, we aim to  clarify some molecular and cellular biology issues such as cell cycle and cell  proliferation, apoptosis, DNA damage and repair, signal transduction,  cytoskeleton dynamic assembly, cell-matrix interactions and nuclear protein  interactions which are related to carcinogenesis. Now we are interested i n a  series of cancer related genes including MARVELD1, c1orf109, SALL4, BI-1, BRI-1,  AIF and some interaction molecules associated with histone  methylation.
2) The  research on the signal transduction of cancer cell
Biological  phenotype of cancer is originated from the disturbance of cell signal  transduction system. To investigate the mechanisms of the aberrant signal  transduction involved i n cell proliferation, apoptosis, adhesion, migration and  invasion, the group focuses on the meaning of the activation and regulation of  Ras family small G proteins including Ras, Rap, Rab ,Rho, Ran and downstream  signal pathway i n various cancer type and i n different pathology phage. We are  intensively investigating the regulatory mechanism of abnormal signal  transduction of cancer, including signaling pathways inside the cells, and on  cell-cell and cell-matrix contacts during the process of the carcinogenesis,  along with the molecular events leading to specific biological phenotype of  cancer. To reveal the physiological and biochemical change of cancer, which  underlies the molecular mechanism of carcinogenesis, cell line model, animal  model and clinic tissue sample were used, and the combined techniques including  protein-protein interaction, protein activation, RNAi, cell immuno-fluorescence  and live cell imaging assays were included as the methodology.
3) The  study of the cancer glycomics and biomarker 
Taking the third  biomacromolecules glycan as the research object, the specific glycan changes i n  serum, cell lines and tissue from the patients of breast cancer,  hepatocarcinomas and pancreatic cancer were studied, based on the improved high  throughput analytical technique of N-glycan. In addition, the relation between  the change of the glycan and the function of glycoprotein was also investigated.  The ultimate aims are to build a rapid, convenient and noninvasive blood  detection technique, by exploring the sensitive and specific cancer biomarker,  and provide new cues to tackle down the target for cancer  therapy.
4) The  study of the cancer metabolomics and biomarker 
On the basis of  developing a research platform of metabolomics with the UPLC-MS/MS, the group  has studied the metabolomics of serum, urine and tissue sample of various  cancers. The goal is to find the micro-molecular metabolomic biomarker of cancer  through the multi-element statistical analysis and to develop the clinical  diagnostic models  for early diagnosis of associated cancer.

Key Publications in recent years:

1.Ming Shi, Shan  Wang, Yuanfei Yao, Yiqun Li, Hao Zhang, Fang Han, Huan Nie, Jie Su, Zeyu Wang,  Lei Yue, Jingyan Cao, Yu Li*. Biological and clinical significance of epigenetic  silencing of MARVELD1 gene in lung cancer. Scientific Reports. 2014; 4: 7545,  doi:10.1038/srep07545.

2.Jiang Q, Wang  J, Wu X, Ma R, Zhang T, Jin S, Han Z, Tan R, Peng J, Liu G, Li Y, Wang Y.  LncRNA2Target: a database for differentially expressed genes after lncRNA  knockdown or overexpression. Nucleic Acids Res. 2014 Nov 15. pii:  gku1173.

3.Xu S, Wu H,  Nie H, Yue L, Jiang H, Xiao S, Li Y*. AIF downregulation and its interaction  with STK3 in renal cell carcinoma. PLoS One. 2014 Jul 3; 9(7):e100824. doi:  10.1371/journal.pone.0100824.

4.Wei L, Liu C,  Kang L, Liu Y, Shi S, Wu Q, Li Y*. Experimental study on effect of simulated  microgravity on structural chromosome instability of human peripheral blood  lymphocytes. PLoS One. 2014 Jun 25; 9(6):e100595. doi:  10.1371/journal.pone.0100595.

5.Jiang Q, Wang  J, Wang Y, Ma R, Wu X, Li Y. TF2LncRNA: identifying common transcription factors  for a list of lncRNA genes from ChIP-Seq data. Biomed Res Int. 2014;  2014:317642. doi: 10.1155/2014/317642.

6.Shi M, Yao Y,  Han F, Li Y, Li Y*. MAP1S controls breast cancer cell TLR5 signaling pathway and  promotes TLR5 signaling-based tumor suppression. PLoS One. 2014 Jan 23;  9(1):e86839. doi: 10.1371/journal.pone.0086839.

7.Jiang Q, Wang  G, Jin S, Li Y, Wang Y. Predicting human microRNA-disease associations based on  support vector machine. Int J Data Min Bioinform. 2013;8(3):282-93.

8.Narla SN,  Pinnamaneni P, Nie H, Li Y, Sun XL. BSA-boronic acid conjugate as lectin  mimetics. Biochem Biophys Res Commun. 2014 Jan 10;443(2):562-7. doi:  10.1016/j.bbrc.2013.12.006.

9.Wei L, Diao Y,  Qi J, Khokhlov A, Feng H, Yan X, Li Y*. Effect of change in spindle structure on  proliferation inhibition of osteosarcoma cells and osteoblast under simulated  microgravity during incubation in rotating bioreactor. PLoS One. 2013 Oct  7;8(10):e76710. doi: 10.1371/journal.pone.0076710.

10.Shan Wang,  Jianran Hu, Yuanfei Yao, Ming Shi, Lei Yue, Fang Han, Hao Zhang, Jie He,  Shanshan Liu, Yu Li*. MARVELD1 regulates integrin β1-mediated cell adhesion and  actin organization via inhibiting its pre-mRNA processing. Int J Biochem Cell  Biol. 2013;

11.Liu X, Nie H,  Zhang Y, Yao Y, Maitikabili A, Qu Y, Shi S, Chen C, Li Yu*. Cell  surface-specific N-glycan profiling i n breast cancer. PLoS One. 2013;  8(8):e72704. doi: 10.1371/journal.pone.0072704.

12.He J, Zhang  W, Zhou Q, Zhao T, Song Y, Chai L, Li Yu*. Low-expression of microRNA-107  inhibits cell apoptosis i n glioma by upregulation of SALL4. Int J Biochem Cell  Biol. 2013; 45(9):1962-73. doi: 10.1016/j.biocel.2013.06.008.

13.Ni S, Hu J,  Duan Y, Shi S, Li R, Wu H, Qu Y, Li Yu*. Down expression of LRP1B promotes cell  migration via RhoA/Cdc42 pathway and actin cytoskeleton remodeling i n renal  cell cancer. Cancer Sci. 2013; 104(7):817-25. doi: 10.1111/cas.12157.

14.Ling S,  Birnbaum Y, Nanhwan MK, Thomas B, Bajaj M, Li Y, Li Y, Ye Y. Dickkopf-1 (DKK1)  phosphatase and tensin homolog on chromosome 10 (PTEN) crosstalk via microRNA  interference i n the diabetic heart. Basic Res Cardiol. 2013; 108(3):352. doi:  10.1007/s00395-013-0352-2.

15.Yan Hongji,  He Yue, Yin Yanbin, Wang Xiumei, Xiongbiao Chen, Song Zhang, Cui Fuzhai, Yu Li,  Yongzhan Nie, Weiming Tian. Self-assembled monolayers with different chemical  group substrates for the study of MCF-7 breast cancer cell line behavior.  Biomedical Materials, 2013; 8(3):035008. doi: 10.1088/1748-6041  /8/3/035008.

16.Xiuping Bai,  Rui Fang, Song Zhang, Xinli Shi, Zeli Wang, Xiongbiao Chen, Jing Yang, Xiaolu  Hou, Yongzhan Nie, Yu Li*, and Weiming Tian*. Self-cross-linkable hydrogels  composed of partially oxidized alginate and gelatin for myocardial infarction  repair. Journal of Bioactive and Compatible Polymers, first published on  February 5, 2013 as doi:10.1177/0883911512473230

17.Qinghua  Jiang, Guohua Wang, Shuilin Jin, Yu Li, Yadong Wang. Predicting human  microRNA-disease associations based on support vector machine. Int. J. Data  Mining and Bioinformatics 2013; 8(3), 282-293.

18.Wang  haochang, Li Yu*. Co-decision Matrix Framework for Name Entity Recognition in  Biomedical Text. International Journal of Data Mining and Bioinformatics.  2013.

19.Wang  haochang, Li Yu*. Transductive Support Vector Machines and Active Learning for  Extracting Protein-Protein Interactions. Journal of Investigative Medicine.  2013; 61(4)

20.Lijun Wei,  Fang Han, Lei Yue, Hongxia Zheng, Dan Yu, Xiaohuan Ma, Huifang Cheng, Yu Li *.  Synergistic Effects of Incubation i n Rotating Bioreactors and Cumulative Low  Dose 60Co γ-ray Irradiation on Human Immortal Lymphoblastoid Cells. Microgravity  Science and Technology: 2012, 24 (5):335-344

21.Huan Nie, Yu  Li* and Xue-Long Sun*. Recent advances i n sialic acid-focused glycomics.  Journal of Proteomics. 2012; 75(11):3098-112.

22.Shan-shan  Liu, Hong-xia Zheng, Hua-dong Jiang, Jie He, Yang Yu, You-peng Qu, Lei Yue, Yao  Zhang, Yu Li*. Identification and characterization of a novel gene, c1orf109,  encoding a CK2 substrate that is involved i n cancer cell proliferation. Journal  of biomedical science 2012; 19:49.

23.Youtao Yu,  Yubao Zhang, Jianran Hu, Hao Zhang , Shan Wang, Fang Han, Lei Yue, Youpeng Qu,  Yao Zhang, Hongjian Liang, Huan Nie, Yu Li*. MARVELD1 inhibited cell  proliferation and enhance chemosensitivity via increasing expression of p53 and  p16 in hepatocellular carcinoma. Cancer Science. 2012;103(4):716-22.

24.Satya Nandana  Narla, Huan Nie, Yu Li*, Xue-Long Sun*. Recent Advances in the Synthesis and  Biomedical Applications of Chain-End Functionalized Glycopolymers. Journal of  Carbohydrate Chemistry, 2012; 31(2):67-92

25.Wenying Mu,  Shanguang Chen, Fengyuan Zhuang, Yinghui Li, Yu Li. Quantification of the  distribution of blood flow pressure with postures. J Biomedical Science and  Engineering( JbiSE), 2012; 5, 113-119 doi:10.4236/jbise.2012.53015

26.Hong-xia  Zheng, Shan-shan Liu, Wei-ming Tian, Hong-ji Yan, Yao Zhang, Yu Li*. A  three-dimensional i n vitro culture model for primary neonatal rat ventricular  myocytes. Current applied physics, 2012; 12(3):826-833.

27.Zheng  Hongxia, Tian Weiming, Yue Lei, Zhang Yao, Yu Li*. Rotary culture promotes the  proliferation of MCF-7 cells encapsulated i n three-dimensional  collagen-alginate hydrogels via activation of ERK1/2-MAPK pathway, Biomedical  materials, 2012; 7(1):015003.

28.Zheng  Hongxia, Tian Weiming, Yan Hongji, Jiang Huadong, Liu Shan-shan, Yue Lei, Zhang  Yao, Han Fengtong, Yu Li*. Expression of estrogen receptor α i n human breast  cancer cells regulated mitochondrial oxidative stress under simulated  microgravity. Advances i n Space Research, 2012; 49(10):1432-1440.

29.Liu SS, Chen  XM, Zheng HX, Shi SL, Yu Li*. Knockdown of Rab5a expression decreases cancer  cell motility and invasion through integrin-mediated signaling pathway. J Biomed  Sci. 2011; 18:58.

30.X. P. Bai, H.  X. Zheng, R. Fang, T. R. Wang, X. L. Hou, Yu Li, X. B. Chen, W. M. Tian*.  Fabrication of engineered heart tissue grafts from alginate/collagen barium  composite microbeads. Biomedical materials. 2011; 6(4):045002.

31.Liu SS, Chen  XM, Zheng HX, Shi SL, Yu Li*. Knockdown of Rab5a expression decreases cancer  cell motility and invasion through integrin-mediated signaling pathway. J Biomed  Sci. 2011; 18:58.

32.Fanli Zeng,  Yanyan Tian, Shuliang Shi, Qiong Wu, Lei Yue, Yao Zhang, Yu Li*. Identification  of mouse MARVELD1 as a candidate microtubule-associated protein that inhibites  cell-cycle progression and migration. Molecules and Cells. 2011;  31(3):267-274.

33.Ning Ding,  Huan Nie, Xuemei Sun, Wei Sun, Youpeng Qu, Xia Liu, Yuanfei Yao, Xue Liang,  Cuiying Chitty Chen, Yu Li*. Human Serum N-Glycan Profiles Are Age and Sex  Dependent. Age and Aging, 2011, 40(5):568-75. doi:  10.1093/ageing/afr084

34.Hongxia Zheng, Weiming Tian, Lei Yue, Lijun  Wei, Yu Li*. (2010) Simulated weightlessness leads to structural aberrant  spindle apparatus and cell cycle arrest i n NIH3T3 fibroblast cells. Space  Medicine & Medical Engineering, 23(6)416-418

35.Shan Wang,Yu Li*, Fang Han, Jianran Hu, Lei  Yue, Youtao Yu, Yubao Zhang, Jie He, Hongxia Zheng, Shuliang Shi, Xiaowei Fu,  Hongjin Wu. Identification and characterization of MARVELD1, a novel nuclear  protein that is downregulated i n multiple cancers and silenced by DNA  methylation. Cancer Letters. 2009; 282:77-86.

36.Fangli Liu, Yu Li, Yang Yu, Songbin Fu and  Pu Li. Cloning of Novel Tumor Metastasis-Related Genes from the Highly  Metastatic Human Lung Adenocarcinoma Cell Line Anip973. Journal of Genetics and  Genomics. 2007; 34: 189-195

37.Li Yu, Qing Chang, Brian P. Rubin,  Christopher D.M. Fletcher, Thomas W. Morgan, Steven J. Mentzer, David  J.Sugarbaker, Jonathan A. Fletcher, and Sheng Xiao. Insulin Receptor Activation  i n Solitary Fibrous Tumors. J. of Pathology. 2007; 211:550-554.

38.Huang JM, Zhao YL, Li Yu, Fletcher JA, Xiao  S. Genomic and functional evidence for an ARID1A tumor suppressor role. Genes  Chromosomes Cancer. 2007; 46:745-750.

39.Li Yu, Jianmin Huang, Yan-Ling Zhao, Ji He,  Wei Wang, Kay E. Davies, Vânia Nosé, and Sheng Xiao. UTRN on chromosome 6q24 is  mutated i n multiple tumors. Oncogene 2007; 26: 6220-6228.